Part:BBa_K4887011

GUS reporter for plant expression

The GUS gene is a commonly used reporter gene. Its expression product beta-glucuronidase is a hydrolase that can catalyze the hydrolysis of many β-glucoside esters. It can decompose X-Gluc into blue substances, to observe the expression of foreign genes in transgenic plants and identify transgenic plants.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1819
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

11.5 Results:

(1) GUS detection for the transgenic plants

The GUS gene, is a commonly used reporter gene harboured in pCAMBIA1301s. Its expression product β-glucuronidase is a hydrolase that can catalyze the hydrolysis of many β-glucoside esters. It can decompose X-Gluc into blue substances, to observe the expression of foreign genes in transgenic plants and identify transgenic plants.
After the regenerated seedlings grew leaves, GUS staining was performed on these transgenic plants. Two successfully transformed sweet potato lines were preliminarily screened and designated as 23216004 and 23216005, whose leaves turned green (Fig. 1).

Fig. 1 Results of GUS staining